Epigenomics
Epigenomics explores modifications to DNA and chromatin that influence gene expression without altering the underlying genetic code. These modifications include DNA methylation, histone modifications, and chromatin accessibility. At Biogrithm, we unravel epigenetic patterns to understand cellular processes, disease mechanisms, and personalized medicine
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Introduction to Epigenomics
Epigenomics explores modifications to DNA and chromatin that influence gene expression without altering the underlying genetic code. These modifications include DNA methylation, histone modifications, and chromatin accessibility. At Biogrithm, we unravel epigenetic patterns to understand cellular processes, disease mechanisms, and personalized medicine.
Services Offered by Biogrithm
1.Bisulfite Sequencing (WGBS / RRBS):
What it is: Bisulfite sequencing detects DNA methylation patterns by converting unmethylated cytosines to uracils.
Methods:
2.Whole-Genome Bisulfite Sequencing (WGBS): High-resolution, genome-wide methylation profiling.
Reduced Representation Bisulfite Sequencing (RRBS): Focused on CpG-rich regions.
Applications: Uncover epigenetic modifications associated with diseases, development, and environmental factors.
3.ChIP-Seq / MeDIP-Seq:
ChIP-Seq (Chromatin Immunoprecipitation Sequencing): Maps protein-DNA interactions, revealing histone modifications and transcription factor binding sites.
MeDIP-Seq (Methylated DNA Immunoprecipitation Sequencing): Identifies methylated regions in the genome.
Biogrithm’s Expertise: We analyze epigenetic marks, unraveling regulatory networks and disease mechanisms.
4.ATAC-Seq (Assay for Transposase-Accessible Chromatin Sequencing):
What it is: ATAC-Seq profiles open chromatin regions, providing insights into gene regulation and enhancer activity.
Benefits: Low input requirements, high sensitivity, and genome-wide coverage.
Applications: Identifying active regulatory elements.
5.Hi-C Proximity Ligation:
What it is: Hi-C captures chromatin interactions, revealing 3D genome organization.
Biogrithm’s Approach: We analyze spatial proximity, uncovering long-range interactions and topological domains.
Single-Cell ATAC-Seq and Single-Cell Multiomics:
Single-Cell ATAC-Seq: Profiles chromatin accessibility at the single-cell level, linking epigenetics to cell
6.heterogeneity.
Single-Cell Multiomics: Integrates transcriptomics, epigenomics, and proteomics from individual cells.
Applications: Understand cellular diversity, developmental processes, and disease progression.
Real-Life Cases
7.Cancer Epigenetics: Identifying aberrant DNA methylation patterns in cancer cells.
Neurodevelopmental Disorders: Unraveling epigenetic changes associated with autism spectrum disorders.
Drug Development: Targeting epigenetic modifications for therapeutic interventions.
Conclusion
Explore the epigenetic landscape with Biogrithm, where we decode hidden regulatory cues!